*Postdoctoral Position: Structural analysis of **Raf-1 and Rok-?
cross-talk***
*Starting date:*As soon as possible
We are seeking a highly motivated post-doctoral fellow to work on a
novel mechanism of intramolecular autoinhibition and cross-talk of
modular kinases Raf-1 and Rok-?. Ras binding to Raf-1 and Rho binding to
Rok-? relieve this autoinhibition and allow the change from a "closed",
inactive conformation to an "open", active conformation. In the "open"
conformation, Raf-1 and Rok-? form a complex in which the regulatory
domain of Raf-1 binds to the kinase domain of Rok-? and directly
inhibits its enzymatic activity (inhibition in trans). This leads to
attenuation of the Rok-? kinase activity by Raf-1.
The aim of the projects is to generate structural information at the
molecular level of Raf-1:Rok-? complexes, and compare it with
Rok-?:Rok-? (Rok-? regulatory domain:Rok-? kinase domain) complexes both
at structural and biochemical level using a combination of structural
biology (in particular X-ray crystallography and SAXS) and modern
biophysical methods, such as ITC, light-scattering, and fluorescence
spectroscopy, together with classical biochemistry.
The individual proteins or their domains planned for this study are
already available in the form of clones or expressed proteins in the
Baccarini laboratory. An integrated approach will be adopted to gain
structural information on target complexes, by using available
structural biology techniques.
The successful post-doctoral applicant will work in K. Djinovic Carugo
group in close collaboration with the FEBS Fellow Dr. Andrea Varga in
the Baccarini group at the Center of Molecular Biology of the University
of Vienna, located at the Max F. Perutz Laboratories. The technical
assistant will work in the Baccarini group.
The Department is equipped to carry out all steps from gene cloning, to
structural analysis including robotics for crystallisation and crystal
imaging, X-ray diffraction, biomolecular NMR facility, and a series of
biophysical and optical spectroscopy techniques (DLS, SLS, ITC, CD,
Thermofluor). The Group has additionally access to major European
synchrotron high brilliance X-ray sources.
*Qualifications and experience: *a Ph.D. in biochemistry, molecular
biology, or a related field, with a strong background in molecular
biology, protein expression/purification, biophysical/biochemical
characterization and macromolecular crystallography techniques are
required. Experience in eukaryotic protein expression and previous
post-doctoral experience in structural biology on macromolecular
complexes are a plus. Good communication skills and organizational
ability will be required. In addition high motivation, the ability to
learn new skills, and good team integration skills are a must*.*
*Contract:*A 3 year contract will be offered to the successful candidate.
*Closing date:*Review of applications begins Dec 15, 2010 and will
continue until the position is filled.
*Web pages:*
http://www.mfpl.ac.at/index.php?cid=58
http://www.mfpl.ac.at/index.php?cid=75
The University of Vienna is an equal opportunity employer.
To apply for this position, please email to Karin Pfeiffer <
*[email protected] <mailto:[email protected]>*>
The application must include a detailed CV with concise description of
research experience and interests; arrange for three letters of
recommendation to be sent to Karin Pfeiffer [email protected]
<mailto:[email protected]>,
****AND****
Sign in the *pool of applicants* at the University of Vienna, following
the instructions at: http://jobcenter.univie.ac.at/applications/?L=2
For the first time registration please go to:
https://univis.univie.ac.at/ebewerber/flow/?_flowExecutionKey=_c24D7C7BB-83ED-E6B6-5FEE-2C33DF9AB336_k1E1895AF-EAF2-6F72-C5E3-9DDB0394DB36
Further information can be obtained from Kristina Djinovic Carugo
[email protected] <mailto:[email protected]>.
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Department for Structural and Computational Biology
Max F. Perutz Laboratories
University of Vienna
Campus Vienna Biocenter 5
A-1030 Vienna
Austria
e-mail: [email protected]
Phone: +43-1-4277-52203/52201
Mobile: +43-664-602 77-522 03
Fax: +43-1-4277-9522
