Hi,
maybe you solved your problem,
if not, you can try this : You prepare your protein at high salt
concentration. After that you add your ssDNA directly and you proceed by
dialysis. You prepare your dialysis with a low salt concentration
buffer. You can use different buffer by step in order to avoid a too
fast salt concentration decrease. This method can allow progressive
assembly of your complex.
Nicolas
Le 31/10/10 08:06, dengzq1987 a écrit :
Hi all,
I want to sovle the structrue of ssDNA -protein complex.but the
protein are unstable at low salt concentration,so we use 1M Nacl in
the buffer. the high salt content may disrupt the complex.and I
don't know which ssDNA lenght try fist.any suggestion or experience
are welcome.
thank you!
2010-10-31
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dengzq1987
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