Here's an ignorant question: When people express an exogenous tRNA in E coli (to overcome rare codon issues, for example, or to supply a cognate tRNA for an orthogonal synthetase), what sorts of promoters are used?
My (ignorant) guess is that something as potent as the T7 promoter might be a waste, since you don't need huge quantities of your tRNA, and you probably don't want to divert resources away from message production for your target gene. But I could be wrong. Any pointers to sequences that successfully direct tRNA production would be welcome, so I could use them as design templates. Thanks, Pat --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. Professor of Biochemistry & Molecular Biology Director, Biochemistry Graduate Program Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA (215) 762-7706 pat.l...@drexelmed.edu
