Dear All,

Could you please provide me some suggestions on following.

1. Could there be difference in interaction between two proteins when produced
 a) both in bacteria,
 b) both IVTT (in vitro transcription and translation), and
 c) one in bacteria and other by IVTT.
Could you please direct  me to some references.

2. I am looking for references where two proteins not interacting by pulldown 
or Native gel analysis (or other assays using purified protein) but protein 
complex crystal structure was solved.

Thanks
Happy Thanksgiving
Yogi


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