Dear colleages,
Currently I met problem in a MR case and hope somebody could advise me.
The crystals belong to sg C2221 with the cell parameters a=59A, b=302A,
c=97A, beta=92.3. The searching model shares 43% identities (300aa) with
target protein. When searching in PHASER program, only one clear solution
could be found with high Z-score: RZF=9.8 and TZF=24.2. However, though the
density is interpretative, the solution coordinate could not fit in the
density (Rfactor high to 54%). Moreover, as suggested by matthews_coff,
there should be at least 2 molecules in one ASU. But I could not find the
other molecules in the ASU in this case (though the other solution are
defined with high Z-score RZF=7.8 and TZF=59, this solution has 115 clashes
with the first one). I also find that this solution coordinate occupied some
"layers" and most of the unit cell are lack of density. As
Phenix.xtrage suggested the data is not twinned, I was totally confused by
this situation.
I will appreciate if you could share your opinions and experience on
similar case with me.
Best Regards,
Xiuwen
