Hi
SAXS can be a right tool. However, how big is "short peptide linker"?
Check Nature paper by Askarieh G. and Hedhammar M. for non-His pH
sensor.
cheers
Alex
Am 06.12.2010 um 17:59 schrieb Daniel Jin <[email protected]>:
Dear CCP4 colleagues,
We have a protein that is composed of two domains connected by a
short peptide linker. We have some indirect evidence showing that
the two domains may somehow move against each other when exposed to
different pH. It is unlikely to have any obvious secondary
structure change since each domain behaves like a rigid body. I am
wondering whether there is any “easy” way, biochemically or biophy
sically, to monitor the conformational changes in solution. Many t
hanks.
As far as I know most of the pH sensing stories are linked to
histidine residue. Can you point me to any references that show a
different pH sensing mechanism (other than His)? Thanks.
Best,
Daniel
