Specific density of protein is about 1.34 g/cm3. Correct by solvent fraction. About 600-700mg/ml for 50% for example. Compute molarity from there.
BR From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Teresa De la Mora Sent: Monday, December 13, 2010 12:08 PM To: [email protected] Subject: [ccp4bb] protein concentration in crystal Hi all I'm looking for a way to calculate the protein concentration in a single crystal. So what I'm thinking is to use the Matthew's number to calculate how many molecules inside the crystal, then multiply that number by Avogadro's number to get moles then divide by volume of crystal. Is this approach correct? Thank you for your advice/suggestion Happy holidays! :) Teresa Teresa De la Mora-Rey Ph.D. Dept. Medicinal Chemistry University of Minnesota 8-101 Weaver-Densford Hall 308 Harvard St. SE, Minneapolis, MN 55455 Lab phone (612) 626-5226 "If you never did you should. These things are fun and fun is good" Dr. Seuss
