The average atomic B factor in the PDB at a given resolution "d" is roughly:

B = 4*d^2+12

So, I am willing to bet your resolution is 3.1 A?  If so, then you are
"normal" (whatever that means).

-James Holton
MAD Scientist

On Wed, Dec 15, 2010 at 8:38 PM, Pavel Afonine <[email protected]> wrote:

> Hi Afshan,
>
> you didn't mention the resolution, which is essential.
> For ultra-high resolution structures the average B may be well less than
> 10, and <B> less than ~4-5 is the condition to see the bonding density (see
> aldose reductase, 1uso, for instance). While a structure at 3.5A resolution
> may have B-factors larger than 100 or so.
>
> Pavel.
>
>
> On Wed, Dec 15, 2010 at 10:24 AM, Afshan Fayazi <[email protected]>wrote:
>
>>
>>
>> **Actually i have sioved my structure of the protein but the B factor
>> (temperature) overall  is very high . it sis more than 50 so how can i
>> reduce this or what is the acceptable B factor for the submission
>>
>>
>> Best Regards
>>
>> AFSHAN
>>
>>
>> <http://de.mc517.mail.yahoo.com/mc/[email protected]>
>>
>>
>>
>

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