Hello: I am making a kinase in e.coli that seems to autophosphorylate itself a bit, which makes it difficult to get a pure homogeneous sample for crystallization. I would like to use a phosphatase to remove the abberant phosphorylation. I have seen lambda phosphatase used for this type of thing in the past, but buying it is pricey. Does anyone have a e coli construct for this or some other phosphatase or perhaps another suggested solution to this dilema? thanks in advance. -Tom
- [ccp4bb] phosphatase construct Brett, Thomas
- Re: [ccp4bb] phosphatase construct Artem Evdokimov
- Re: [ccp4bb] phosphatase construct Hari Namboodiri
