I vaguely remember a paper by Aled Edwards, where they did the statistics for their targets at SGC. As far as I recall they engineer with cleavable tag purify and cyrstallize it first with tag and only if they don't get crystals or a structure they cleave off the tag and rerun the screens. I think the numbers where 30% of the proteins have been cleaved.
Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/<http://web.me.com/bosch_lab/> On Jan 7, 2011, at 9:40 AM, Snijder, Arjan wrote: Dear all, Best wishes for the new year! I am looking for some statistics about the fraction of protein structures in the pdb that are actually crystallized with a His tag, and from those crystallized with a His tag whether these construct were engineered with a cleavable tag or not. Has anybody done such an analysis and would want to share the outcome? In the mean time I will mentally prepare myself for re-freshing my perl knowledge to write a script to do such an analysis. Any suggestions on how to quickly analyse this would be appreciated. cheers Arjan Arjan (Harm Jan) Snijder Associate Principal Scientist Astra Zeneca R&D, Mölndal Discovery Enabling Sciences and Capabilities, DECS SE-431 83 Mölndal Sweden tel: ++46 31 7763691 [email protected]<mailto:[email protected]> ________________________________ Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful.
