Hello Qing Chen, if by 'shift' you mean the actual coordinates you can do in coot as Herman describes. If you want to shift the sequence numbering: Coot also has an option to renumber a sequence by an offset and the offset can also be negative.
Cheers, Tim On Thu, Jan 13, 2011 at 12:27:49PM +0100, Qing Chen wrote: > Let's say I need to insert two G between resi 98 and 99. I tried in the way > as Herman suggest: break the chain in the domain boundary, add terminal > residues at the N-terminus of the 2nd domain. But then there is no space to > accommodate the inserted residues, the two glycerin backbones overlap with > resi 97 and 98. Is there a way to shift the whole 1st domain a bit ? > > > > On Thu, Jan 13, 2011 at 12:16 PM, <[email protected]>wrote: > > > use coot: calculate - model/fit/refine - add terminal residue. > > this will add alanines. you can change these to glycines with the simple > > mutate option in the same pop up menu. > > > > best, > > HErman > > > > ------------------------------ > > *From:* CCP4 bulletin board [mailto:[email protected]] *On Behalf Of > > *Qing > > Chen > > *Sent:* Thursday, January 13, 2011 11:58 AM > > *To:* [email protected] > > *Subject:* [ccp4bb] creat a model with insertion > > > > Dear all, > > > > I have the wt protein structure that contains two domains. I want to creat > > a model with two glycerin inserted between the two domains. > > Which software or webserver could do this? > > Pls help. > > > > > > Qing Chen > > > > -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
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