The way to go is make-your-own, especially since it is a pretty lousy enzyme (we often use 1:5 to 1:10 molar ratio of TEV:protein). You can get expression vectors here:
http://mcl1.ncifcrf.gov/waugh_tev.html Bert ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Gloria Borgstahl [gborgst...@gmail.com] Sent: Friday, January 14, 2011 9:36 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] TEV protease We are using TEV protease to make a big batch of protein for structural studies. We usually use thrombin, so this is our first time using this enzyme on a large scale. Boy is it expensive! Does anyone know of a bulk source for this enzyme and what ratios of use do you recommend. Imidazole inhibits? Many thanks. Gloria
