Dear Chandan, 388 buffers are quite a bit. I presume you are talking about intervals of pH and not 388 buffers all at one pH. Can you explain a bit more about how you determined that the buffer is the problem, and what results led you to that conclusion?
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of chandan kishore Sent: Thursday, January 27, 2011 1:43 AM To: [email protected] Subject: [ccp4bb] buffers for crystallization Hi Everyone, I have to crystallize one protein which contains a zinc fingure motifs, I am facing a problem in crystallization. I already used 388 buffers . Can anyone suggest me some books or papers freely available online on Buffers required for crystallization. Thanks -------------------------------------------------------------------------- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful.
