Dear all,
Thanks for your hearty responses. Below is a summary for the
information I received. Again, I really appreciate all the help.
Best regards,
Junyu
1. Yale University, University of Utah, and Wake Forest University are
three that have core facilities which perform N-terminal sequencing.
2. A colleague here has had good results with the facility at Iowa
state:
http://www.biotech.iastate.edu/service_facilities/protein.html
If you just want to identify the protein, mass spec may be cheaper.
The same place will do that, or we have had excellent results with
http://www.appliedbiomics.com/
If you need to confirm N-term construct, Edman degradation is probably
still more cost
effective.
3. I've gotten decent results from Rockefeller:
http://proteomics.rockefeller.edu/edman/edmaninfo
4. We have used Alphalyse (http://www.alphalyse.com/picknpost.html).
5. There is one at the Johns Hopkins University.
Synthesis and Sequening facility
6. You can get it here as well...
University of Pittsburgh
Department of Biological Sciences
Fifth and Ruskin Aves
Clapp Hall 301
Pittsburgh PA 15260
email: [email protected]
ph: 412.624.0106
or
Jack Presley
Laboratory Manager
UC Davis
Molecular Structure Facility
http://msf.ucdavis.edu
(530) 752-7327
7. Proteos
4717 Campus Drive
Kalamazoo, Michigan 49008
269.372.3423
http://www.proteos.net
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Junyu Xiao, Ph.D.
University of California, San Diego
Leichtag Room 283
9500 Gilman Drive, 0721
La Jolla, CA 92093-0721
Lab phone: 858-822-0684
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