http://www.ysbl.york.ac.uk/~alexei/molrep.html#stick
On Thu, 2011-02-17 at 19:32 +0100, Christian Roth wrote:
> Dear all,
>
> I tried MolRep with a two domain protein. I have cut the two domain as one
> domain rotates which prevent a search with the complete model. After I
> finished
> the first run. I put this solution as fixed input model in the second molrep
> run
> with the second domain. The resulting solution positioned the tow expected
> molecules, but not nearby the two already found domains. Interestingly a
> symmetry mate would be at the correct position. I could manually write out
> the
> coordinates of the symmetry molecule to put the two domains together, but I
> thought Molrep would position it close to the already found domains because
> they belong together and in fact they are one polypeptide chain.
> Why does this not happen? Do I have to incorporate additional information or
> constrains for the Molrep run?
>
> Thanks and Best Regards
>
> Christian
--
"I'd jump in myself, if I weren't so good at whistling."
Julian, King of Lemurs
