Hi Careina,

Just an example of a pir file which I just generated (using Bart Hazes program mcfman):

P1;>MALDH_
Just a title
TKVSVVGAAGTVGAAAGYNIALDIADEVVFVDIPDKEDDTVGQAADTNHGIAYDSNTRVR
QGGYEDTAGSDVVVITAGIPRQPGQTRIDLAGDNAPIMEDIQSSLDEHNDDYISLTTSNP
VDLLNRHLYEAGDRSREQVIGFGGRLDSARFRYVLSEEFDAPVQNVEGTILGEHGDAQVP
VFSKVSVDGTDPEFSGDEKEQLLGDLQESAMDVIERKGATEWGPARGVAHMVEAILHDTG
EVLPASVKLEGEFGHEDTAFGVPVSLGSNGVEEIVEWDLDDYEQDLMADAAEKLSDQYDK
IS*

I don't think the pir format has changed much since the subroutine that extracts the sequence from a coordinate file was written. As you will see the (ASCII) format is pretty simple, one start record, one title record followed by records containing the sequence in the one-character abbreviation, 60 characters per line, with "*" to end the sequence. The start record is P1;> followed by 6 characters.

I never had any problems with the pir files generated by mcfman, and I always have used an editor (vi is the one I use) to convert other formats to this format.

Fred.

Careina Edgooms wrote:
Dear CCP4 mailing list

I have a relatively simple question. How do I get sequence file in .pir format which is required for many programs? I normally use fasta format but some programs eg arpwarp do not allow me to use that

Thanks for your help

Careina

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