Dear Harvey, I had very good results with low melt agarose and the detergent screen (e.g. Hampton Research). In both cases, the tendency for cluster formation as well as the diffraction power could be improved.
Anja > Dear CCP4BBers, > > Recently I got a crystal which appeared in the condition 2.1M DL-Malic > Acid, > pH 7.0. The crystal looks like a dumbbell but was composed of a cluster of > very thin needles as shown in the picture. The crystal can be repeated but > the diffraction is poor. I have tried the grid optimization by pH and salt > concentration gradients, however there was no improvement in either the > shape or the quality of the crystal. Does anyone have some experiences in > optimizing this kind of crystals? Any suggestion is appreciated! > > Harvey > Anja Pomowski Universität Freiburg Institut f. Organ. Chemie und Biochemie AK Prof. Einsle Albertstr. 21 79104 Freiburg Tel. 0761 2036088 Fax. 0761 2036161
