Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, et al. 2009 Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag. PLoS ONE 4(12): e8119. doi:10.1371/journal.pone.0008119
Unless the protein in question happen to be Leu as a C-terminal residue, this tag won't give wild type C-terminus after cleavage.
- Dima
