Dear Hua, adding water as suggested by Jan Kern could also be accomplished in a more sophisticated way by using dialysis buttons. They require large volumes, though, 5mul is the minimum as far as I know.
At the fancy end of this you could try the TOPAZ system by fluidigm. At the ECM in Darmstadt one of the companies presented a cheap, plasitc based version of this which looked quite appealing to me. Maybe it was the CrystalHarp from Molecular Dimensions, but I am not sure. Cheers, Tim On Sat, Feb 26, 2011 at 09:13:49PM -0500, Hua Yuan wrote: > Dear CCP4 community members, > > I've been trying to crystallize a protein complex that's very sensitive to > ionic strength, i.e., lower salt (~0.3M) will cause precipitation of the > complex but higher salt (~0.5 M) breaks the complex apart. The interaction > that holds the complex is probably mainly ionic type. > The crystals I got so far has only one component of the complex from which > all the crystallization conditions have high salt such as 2M Ammonium > Sulfate in them. Besides repeatly screening many crystallization > conditions, I was wondering whether is any way to work around this problem. > Your suggestions would be greatly appreciated! > > Thanks, > > Hua -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
signature.asc
Description: Digital signature
