Hi Sebastiano,
we have used both silica based and superdex columns and we found the
second as a better option for general usage.
Most of our proteins are on basic buffer and about 50% of them stick on
the silica based column. On the other side a superdex 200 10/30 from GE
performed quite well, we got quite accurate results for both a ~250kDa
hexameric enzyme as also for a 0.3 mg/ml injection of an insulin sample.
Eventually I think depends on the applications you prefer to have.
cheers
Nikos
Dr Nikos Pinotsis
Max F. Perutz Laboratories
University of Vienna
Campus Vienna Biocenter 5
A-1030 Vienna
Austria
Phone: +43-1-4277-52224
On 03/08/2011 02:03 PM, Sebastiano Pasqualato wrote:
Dear all,
I was wondering if somebody could help me out by suggesting the "best" column
to be used in a Static Light Scattering (I guess it would be the same for a Multi Angle
Light Scattering) instrument.
We were suggested using a silica-based column, with very high separation
properties, but it seems that these columns are highly sensitive to (even
slightly) basic pH's. Even running the column in PBS, it looks like injecting
samples at pH 8.0 ruins the column resin, making it unusable.
On the other hand, GE Healthcare columns would require a huge amount of
material to be loaded.
What are you guys using in your instruments?
Thanks a lot in advance for the feedback,
best,
ciao
Sebastiano
