I would certainly try cocrystallizing with adp, amppnp, or ATPgS Many helicases have been crystallized in this way. Papers by Sawaya et al and Singleton et al come readily to mind.
Best of luck Savvas On 09 Mar 2011, at 15:52, Ed Pozharski <epozh...@umaryland.edu> wrote: > On Wed, 2011-03-09 at 10:52 +0000, Art wrote: >> No protein crystal was found after some typical conditions (kit I, kit >> II, and Index) were repeatly screened for more than three times. > > See Einstein's definition of insanity :) > >> Protein concentration gradient was considered, but it can not work. > > Not sure what you mean by this, but you can also try diluting reservoir > solutions. Also, given your protein buffer, you may be more or less > locking yourself into a narrow pH range - try 10mM MES instead. > > -- > "I'd jump in myself, if I weren't so good at whistling." > Julian, King of Lemurs
