Hi Ray; In case of my protein 5%-10% of glycerol help to increase the solubility both of the soluble domain as well as the trans-membrane domain,improve crystal quality dramatically and even prevent radiation damage to some extent.
Best of luck Bashir On Thu, March 10, 2011 23:04, Ray Brown wrote: > Hi all, > > I was intrigued by the recent question of whether glycerol had any adverse > effects on the final purity of protein isolated by chromatography. > Glycerol > certainly helps to solubilize some proteins. Does anyone know of any > negative > effects of glycerol in protein purification, on protein crystal quality or > use in cryocrystallography and on X-ray diffraction results? > > Cheers. > > Ray Brown -- Muhammad Bashir Khan ************************************************** Department for Structural and Computational Biology Max F. Perutz Laboratories University of Vienna Campus Vienna Biocenter 5 A-1030 Vienna Austria Austria Phone: +43(1)427752224 Fax: +43(1)42779522
