1) Make your own with in vitro transcription (straight T7 r T7+RDRP like in Finnzymes kit) 2) Buy from IDTDNA - they are very good
Long RNA tends to be expensive. Consider RNA ligase if two or more pieces can be stitched together. Artem On Sun, Mar 13, 2011 at 3:39 AM, Michael Thompson <[email protected]>wrote: > Hello All, > > I am looking for some advice from some experienced RNA crystallographers. I > would like to order some relatively short (<90 bases) synthetic RNAs for > crystallization trials. I was wondering if anyone could comment on the use > of synthetic RNAs for crystallization. Specifically, what is the longest > synthetic RNA that can be used for crystallization trials? I've seen some > structures in the PDB that are up to 88 bases and are reported to have been > obtained with synthetic constructs (3OWI - glycine riboswitch), but I don't > really know if that's routine or if it's an exceptional case. Also, for > those who have experience with the use of synthetic RNAs, I was wondering > where people generally order their synthetic constructs from? Our resident > expert in RNA crystallography recommended a company called Dharmacon (part > of ThermoFisher), but I was hoping that I might get some other opinions as > to which companies make the best quality oligonucleotides, provide samples > with the highest purity, and have the most reasonable prices. > > Thanks in advance for the help! > > Mike > > > > -- > Michael C. Thompson > > Graduate Student > > Biochemistry & Molecular Biology Division > > Department of Chemistry & Biochemistry > > University of California, Los Angeles > > [email protected] >
