>
>
> hi sreetama,
>
if ur protein is making aggregates during
crystallization try to add glycerol, EDTA in Ur buffer. R u purifying ur
protein from Gelfiltration column ?is ur protein is coming in proper place?
if every thing is right then u can try DTT and TCEP in ur buffer instead
of Beta mercaptoethanol.
for one of my crystallization experiment i used 1- 10 mM of Beta
mercaptoethanol.
--
vandana kukshal
CSIR-senior research fellow
X ray lab ,MSB division
central drug research institute
lucknow
--
Vandana Kukshal
Postdoc Fellow
structure biology group
international Center For Genetic Engineering and Biotechnolgy
Aruna Asaf Ali Marg
110 067 New Delhi
INDIA
