Just like you would for PCR: - Dissolve the oligonucleotide in water - Heat it to above meltung temperature, pick 95degree C if you want to be on the save side - leave it to cool down.
Is this what you are looking for? Tim On Thu, Mar 17, 2011 at 07:23:07PM +0800, dengzq1987 wrote: > Dear all, > > Recently,i purchase Oligonucleotide from company.the sequence is > TTTTTTTTTTGCGTAC GCAC GTACGC .i want to perform self-annealing process to > form the following second structure . > 5' TTTTTTTTTTGCGTACGC > ||| ||| ] > 3' CGCATGCA > > i hope that most of the Oligonucleotide can form this structure,how can i > achieve this? any adivice is appreciated. > > > > best wishes. -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
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