Dear All, Does anybody know of examples where labile protein complexes have been crystallized after using non-specific crosslinkers (so excluding complexes with engineered SS bonds, for example)?
Thanks in advance for any examples, Bert On 3/23/11 10:19 AM, "Parthasarathy Sampathkumar" <spart...@gmail.com> wrote: Dear Bert, You could try the program Hole from http://www.ncbi.nlm.nih.gov/pubmed/9195488 J Mol Graph. <http://www.ncbi.nlm.nih.gov/pubmed/9195488> 1996 Dec;14(6):354-60, 376. HOLE: a program for the analysis of the pore dimensions of ion channel structural models. Smart OS <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Smart%20OS%22%5BAuthor%5D> , Neduvelil JG <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Neduvelil%20JG%22%5BAuthor%5D> , Wang X <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Wang%20X%22%5BAuthor%5D> , Wallace BA <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Wallace%20BA%22%5BAuthor%5D> , Sansom MS <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Sansom%20MS%22%5BAuthor%5D> . -Partha On Wed, Mar 23, 2011 at 9:02 AM, Van Den Berg, Bert <lambertus.vandenb...@umassmed.edu> wrote: Hello all, Does anyone know how to get values for pore sizes of membrane channels? I'm not interested in A x B angstrom values measured between atom centers and assuming a regular pore shape, but a "real-life" value of either surface area at the narrowest point or the volume of a block centered on the narrowest point of the pore. Thanks, Bert
