Hi,
we recently published a method that could be just what you need, and
easy to implement.
It detects covalent modifications of cysteines, and mercury, gold and
some platinums target cysteines...
have a look at this paper.
feel free to contact me if you want more information.
vincent
Fluorescence Detection of Heavy Atom Labeling (FD-HAL): A rapid method
for identifying covalently modified cysteine residues by phasing atoms.
Chaptal V, Ujwal R, Nie Y, Watanabe A, Kwon S, Abramson J.
J Struct Biol. 2010 Feb 10.
Le 3/25/11 8:39 AM, Careina Edgooms a écrit :
Dear ccp4 users
I would like to know, is there a way to check that heavy metal has
bound to crystals before I take it to synchrotron which is far away?
thanks
Careina
--
Vincent Chaptal, PhD
Institut de Biologie et Chimie des Protéines
Drug-resistance modulation and mechanism Laboratory
7 passage du Vercors
69007 LYON
FRANCE
+33 4 37 65 29 01
http://www.ibcp.fr
