We had a case with a BME adduct clearly seen in the structure. PDB ID 1CY5.
Leemor On 4/15/11 4:09 PM, "Michael Thompson" <[email protected]> wrote: > Hi All, > > I was wondering if anyone knew whether or not it is possible for reducing > agents with thiol groups, such as DTT or beta-mercaptoethanol (BME), to form > covalent S-S bonds with Cys residues, particularly solvent-exposed Cys? I have > some puzzling biochemical results, and in the absence of a structure (thus > far), I was wondering if this might be something to try to control for. I have > never heard of this happening (or seen a structure where there was density for > this type of adduct), but I can't really think of a good reason for why this > wouldn't happen. Especially for something like BME, where the molecule is very > much like the Cys sidechain and seems to me like it should have similar > reactivity. The only thing I can think of is if there is a kinetic effect > taking place. Perhaps the rate of diffusion of these small molecules is much > faster that the formation of the S-S bond? > > Does anyone know whether or not this is possible, and why it does or does not > happen? > > Thanks, > > Mike > > >
