Hi all, I am trying to decide between using a N-term his-tag or an N-term flag tag to be expressed on a protein that I will eventually want to try and crystallize. I usually don't cleave my tags unless absolutely necessary and I want to avoid double tagging. I am leaning towards the flag since from my experience this tag is good for protein interactions (pulldowns), but I don't know the effects of a flag tag in crystallization trials. Does anyone have experience with crystallizing protein with flag tags?
Thanks, Christine
