Hi Nisha, I think there is no problem for you B value. The wilson B is just a estimation. For your data, it is acceptable. Best, Jiamu
On Sat, Apr 23, 2011 at 2:41 AM, nisha pandey <[email protected]> wrote: > Dear ccp4bb, > I want to know regarding Wilson B value and mean B-value of protein after > refinement, In my case there is large difference in Wilson plot and > overall.Is it OK to have such value ie Wilson B-value 42 and mean B value > is 23.3 ? > Also in the same structure I have observed that in a few residues B-value > of main chain are higher than that of side chain.It seems quit unusual > .Kindly help me sort out this problem. > Some basic information regarding the data are given below. > > Resolution range 41.24-2.7 > Rsym -15 > I/Sigma-17.2 > I/Sigma -4.2 > (In highest resolution shell) > Rfactor -21.4 > R free -24.2 > > Thanks in advance > > With regards > Nisha > -- Jiamu Du, Ph.D. Postdoctoral Research Fellow Laboratory of Structural Biology Memorial Sloan-Kettering Cancer Center RRL 269, 430 E 67th Street New York, NY, 10021 E-mail: [email protected] Tel: (217) - 417 - 9897
