This intertwined conformation is very possible and is reminiscent of Trp
repressor (at least in the view you provided).
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
Phone: 972-8-647-2220 ; Fax: 646-1710
Skype: boaz.shaanan
________________________________
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Ramanuj
Banerjee
Sent: Tuesday, April 26, 2011 10:37 AM
To: [email protected]
Subject: [ccp4bb] problem with pdb & its symmetry molecule
Dear All, I have solved a protein structure (experimentally phased) with 1
molecule in the asymmetric unit at 2.22 A (high resolution). The present R
factor is .22 and R free .27 with Ramachandran favoured >98% and R and R free
are decreasing with refinement.The problem is: when the pdb is opened in pymol
and symmetry mates generated, the upper part of the molecule shows to be
intertwined with the symmetry molecule (attached .jpg), but there are no
clashes in between the two.The electron density is so very fine that no
alternative choices of chain flow are available.All the processes starting from
phasing and refinement have been done in Phenix.Is such a thing possible ?
