We actually screen with unpurified oligos (up to just over 30nt) and it works just fine. Saves lots of time & $$. If we get hopefull crystals, we pay for purification or do it ourselves by gel. Sometimes it makes the crystals better and sometimes it doesn't. Phoebe
===================================== Phoebe A. Rice Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 http://www.rsc.org/shop/books/2008/9780854042722.asp ---- Original message ---- >Date: Thu, 28 Apr 2011 08:32:23 -0500 >From: CCP4 bulletin board <[email protected]> (on behalf of "Wallen, >Jamie" <[email protected]>) >Subject: [ccp4bb] DNA oligonucleotide purification >To: [email protected] > > All, > > Our laboratory is looking for a new column to purify > short (40mers or less) DNA oligonucleotides that > will be used for crystallization setups. I wanted to > ask if anyone has suggestions of a column that will > give excellent separation of N and N-1 products. We > have a Shimadzu HPLC system. We are considering > Dionex columns as well as Oligonucleotide Separation > C18 columns from Waters, but if there are others > that are better we would like to know. Any > suggestions will be greatly appreciated. Thank you! > > Jamie Wallen
