[ccp4bb] crystal bent once open cover slip

[weikai]

Hi Folks,

We have some membrane protein crystals that are grown in 30%PEG400, 
0.1M Na Citrate pH 4.5, 0.1M LiCl. The protein is purified in DDM. The 
crystals are long rods and grown under room temperature in a hanging 
drop set up.  But once we open the cover slip, we see the rods start to 
break and bend in a few seconds.  Since it is in high PEG400, we just 
directly freeze the crystals.  The diffraction only goes to 10 Ang at 
synchrotron.  Have anybody had similar problem before and any 
suggestions?

Thanks a lot,

Weikai