You could also consider organic solvents (or a mix) for crystallisation
trials too. If you scan through the literature you will find that small
peptides have in the past been treated as small molecules in terms of
crystallization. Once the peptide gets over say 20 amino acids (not the
exact number) then the peptides are treated more as small proteins for
crystallography. On a side I did just see that the structure of Lysozyme
in 50% ethanol was deposited in the PDB (not that lysozyme is a small
peptide).
Good Luck!
Gina
-----Original Message-----
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of
Buz Barstow
Sent: Wednesday, May 25, 2011 8:04 AM
To: [email protected]
Subject: [ccp4bb] Peptide Crystallization
Dear all,
I am considering trying to crystallize a small peptide (around 15 amino
acids). The peptide is soluble in neutral water or buffer (pH 7.0) until
at least 10 mM (13 mg/mL), and adopts a turn conformation when bound to
Zn.
What are your thoughts on attempting this?
If you think that it is worthwhile, what crystallization conditions
would you try? I am thinking of a sparse matrix screen using the Hampton
Crystal Screen 1 and 2 kits, using hanging drop crystallization in
Hampton Vdx trays.
Thanks! and all the best,
---Buz
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