Dear All, I have a severe prob lam to performed my ligand binding study with corresponding protein. I have taking the native diffraction data at 1.75 A and after that i have performed soaking as well co-crystallization experiment with my inhibitors. Problem is that at the active site phosphate ion always bind instead of inhibitors. I have used 1.6 M ammonium phosphate conc at the crystallization recipe which is a very weak inhibitor of my protein whereas the ligand is already clinically applicable but due to the very high conc. of phosphate i have not achieved my target. If some one can suggest me what else i can replace with ammonium phosphate or any other suggestions would be appreciated.
I have tried to grown crystals some other condition but the crystal was not diffracted beyond 3.5 A. Best Regards AFSHAN
