Thanks a lot! I did find some map statistics (average, standard deviation...). Tt also provide a map dimension, where I think we can covert the contour level to X-ray sigma level.
Thanks again for the information. Hailiang > Try here: > http://www.ebi.ac.uk/pdbe/emdb/ > You can (if you get the EM map) play around with various programs and > manipulate the level until the mask matches the molecular weight of your > object of interest. > Or contact the authors of the EM map. Ask for either CCP4 format map or > MRC, you can use programs from the USF suite to inter convert between the > formats. If they used EMAN then most likely the CCP4 format is still bogus > but MRC works fine. > > Jürgen > > On Jun 2, 2011, at 5:24 PM, Hailiang Zhang wrote: > > Hi, > > I am trying to compare a published EM map with X-ray map in hand, and have > several questions: > > 1. EM map seldom indicates the sigma level, and it was said because of the > box size uncertainty during EM model construction. Now, I wonder is there > any way we can sort of its equivalent sigma level to X-ray map? > > 2. The EM structure deposited in pdb don't have any experimental data, and > not sure how to obtain them and generate the map file. > > Sorry if this is wrong place to post EM questions. > > Hailiang > > ...................... > Jürgen Bosch > Johns Hopkins Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > Johns Hopkins Malaria Research Institute > 615 North Wolfe Street, W8708 > Baltimore, MD 21205 > Phone: +1-410-614-4742 > Lab: +1-410-614-4894 > Fax: +1-410-955-3655 > http://web.mac.com/bosch_lab/ > > > >
