Dear Atul, Whilst finding Se-Met conditions you could always just phase with mercury if you are lucky enough to have an accessible free Cys residue or try NaI or I3C soaks. Always worth trying if you have readily reproducible crystals…
Best of luck Marc From: atul kumar <atulsingh21...@gmail.com<mailto:atulsingh21...@gmail.com>> Reply-To: atul kumar <atulsingh21...@gmail.com<mailto:atulsingh21...@gmail.com>> Date: Mon, 13 Jun 2011 15:53:12 +1000 To: "CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>" <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: [ccp4bb] Fwd: Regarding Sel-Met containing proteing crystallisation ---------- Forwarded message ---------- From: Dilip Kumar <dku...@igib.in<mailto:dku...@igib.in>> Date: Mon, Jun 13, 2011 at 11:21 AM Subject: Fwd: Regarding Sel-Met containing proteing crystallisation To: atulsingh21...@gmail.com<mailto:atulsingh21...@gmail.com> ---------- Forwarded message ---------- From: Dilip Kumar <dku...@igib.in<mailto:dku...@igib.in>> Date: Sat, Jun 11, 2011 at 6:09 PM Subject: Regarding Sel-Met containing proteing crystallisation To: CCP4BB@jiscmail.ac.uk<mailto:CCP4BB@jiscmail.ac.uk> Dear all I have got protein crystals,crystallisation condition (LiCl, PEG and HEPES) .Crystals of native protein have been successesfully reproduced but when i tried to reproduce these crystals with protein having Met replaced by Sel-Met, i could not get any crystal.I tried crystallisation trials by varying pH and PEG concentration and diffferent drop ratio but i could not get any hit.Please suggest me what could be the possible reasons behind it? And also suggest the other variables that i can try ? thanks With Regards -- Dilip Tiwari Graduate Student Structural Biology Unit Institute of Genomics & Integrative Biology Delhi-07 -- Dilip Tiwari Graduate Student Structural Biology Unit Institute of Genomics & Integrative Biology Delhi-07
