Based on the dents in my own forehead from banging it on similar brick walls, it would probably be more efficient to get a heavier atom with fewer binding sites, and work your way in from there. If your data set really only extends to 6A, even when you do find all those Se atoms their phasing power may be pretty disappointing.
Phoebe ===================================== Phoebe A. Rice Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 http://www.rsc.org/shop/books/2008/9780854042722.asp ---- Original message ---- >Date: Wed, 15 Jun 2011 16:36:59 +0300 >From: CCP4 bulletin board <[email protected]> (on behalf of tommi kajander ><[email protected]>) >Subject: [ccp4bb] low res. SAD phasing >To: [email protected] > >Dear all, > >Does anyone have suggestions for 6 Å resolution phasing with large >number (40-50) Se sites (SAD so far)?? > >Thanks a bunch, >Tommi > >Tommi Kajander, Ph.D., Docent >Macromolecular X-ray Crystallography >Research Program in Structural Biology and Biophysics >Institute of Biotechnology >P.O. Box 65 (Street: Viikinkaari 1, 4th floor) >University of Helsinki >FIN-00014 Helsinki, Finland >Tel. +358-9-191 58903 >Fax +358-9-191 59940
