In addition to Fred's suggestion, are you certain about your space group ? I assume you tried phaser with different space groups turned on ? Another thing to look at maybe the model you are using for MR has some extensions that lead to clashes in your crystal packing and therefore the second molecule can not be placed. You can trim your model to a core and try searching with that. Or if your one molecule is correctly placed do some rigid body refinement, then use this solution as fixed input model for molrep and try placing the second one. How does your selfrotation function look like ? Jürgen
...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Jun 22, 2011, at 5:08, Afshan Begum <[email protected]> wrote: > Dear all, > > Could any one help me regarding my serious problem actually i have collected > data at 3.0 and cut off 3.1 where the data statics showed the good values > for the further processing. > According to the methew coefficient there would be two molecule in the > asymmetric unit but after running the molrep its provide only one monomer > instead of two, for this reason R values is very high 50%. Actually > homologous model having P6322 space group where as my one is F4132. I had > tried to run phaser as well phenix but both were failed to process further. > i really do not know how can i get the second chain in my structure. Please > if you have some ideas i will appreciate and would be many thankful to you. > > Hope to hearing you soon > > Best Regards > > AFSHAN > >
