Amonium sulfate precipitation? Considering the fact that you tried to seperate tubulin contamination both by charge and mass difference amonium sulfate pption may work.
Shiva On Thu, Jun 23, 2011 at 4:05 AM, Seungil Han <[email protected]> wrote: > All, > I am sorry that this is off topic. > My target protein expressed in insect cell expression system is eluted with > tubulin contamination. We have tried ion exchange & sizing column to get > ride of tubulin from our target protein for structural studies. > > Has anyone had similar problem? Any suggestion? > > Thanks in advance. > Seungil > > > Seungil Han, Ph.D. > Pfizer Inc. > Eastern Point Road, MS8118W-228 > Groton, CT 06340 > Tel: 860-686-1788, Fax: 860-686-2095 > Email: > [email protected]<http://us.mc806.mail.yahoo.com/mc/[email protected]>
