Please clarify: Did you process the sets separately from the images?
Or did you process 360 degrees of images all the way to scaled, but unaveraged results, then average reflections from the 5 different rotation ranges? Or something else? With most processing programs that I am aware of, one can adjust their sigmas to anything they want to. What happens when you use the same adjustments for the 180 degrees that you did for the others? -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Vennila Natesan Sent: Sunday, July 10, 2011 7:54 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] abnormal I/Sigma over phi rotation range Dear CCP4BB users, In order to determine the redundancy at which the structure can be solved, I divided the master data set of my protein into five sets with phi rotations of 45,65,90, 180 and 360 degrees. I got the mean I/Sigma value as 22.4(11.4), 21.7(11.0), 22.7(11.2), 60.9(45.2) and 15.6(8.1) respectively. I will be very helpful if i get any idea/possible reasons for the abnormal value for 180 degree dataset. For the information, the completeness values are 75.7(78.5),92.6(92.7),99.5(97.7),99.6(97.7)and 99.5(96.4) respectively and values inside brackets are for highest resolution shell. There was no noticable change in mosaicity value. The Rmerge values are 2.2, 2.4, 2.7, 3.2, and 5.7 (8.7) respectively. Thanks in advance
