Seconding David's suggestion, I have had this issue on several occasions and I would highly recommend using SHARP. In my experience, SHARP does a superior job handling this type of data. Best of luck-
Steve ________________________________ From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of David Veesler Sent: Tuesday, July 12, 2011 9:21 PM To: [email protected] Subject: Re: [ccp4bb] how to combine the experimental phase and molecular replacement phase Hi Jiamu, to complete the Juergen answer, that's true that the MR-SAD approach is very powerful and can definitely help in your case. You can use Sharp and add the information from your partial molecular replacement solution encoded as HL coefficients and do a MR-SAD phasing. Here is the procedure http://www.globalphasing.com/sharp/manual/chapter4.html#ExternalPhaseInformation Cheers David Le 12 juil. 2011 à 18:08, Jiamu Du a écrit : Dear All, I am now working on a low resolution phase determination (around 3.3 A with Se anomalous signal around 3.8 A). I can find the Se site and get the phase, but the density map is not so good. Some part of the protein (about 1/3) has a homologue model which is also can be found using Phaser. The homologue region has a good map while other region only show a poor map. I think the combination of experimental phase and MR phase might improve the map. Is there anybody can help find which program can work on this? Thanks. -- Jiamu Du, Ph.D. Postdoctoral Research Fellow Laboratory of Structural Biology Memorial Sloan-Kettering Cancer Center RRL 269, 430 E 67th Street New York, NY, 10021 E-mail: [email protected] Tel: (217) - 417 - 9897 -- This message has been scanned for viruses and dangerous content by MailScanner <http://www.mailscanner.info/> , and is believed to be clean. -- This message has been scanned for viruses and dangerous content by MailScanner <http://www.mailscanner.info/> , and is believed to be clean. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
