On Thu, 14 Jul 2011, Wonjin Bae wrote:
Hi, all
Recently, some bactrial source enzyme(sialidase) was subcloned pET20b and
pGEX4T3 vector.
Then, these two construct were transformed to BL21(DE3) expression host.
DNA sequencing results were accurate.
In case of pGEX, many colony was formed and shown the high level of expression.
But, colony was not shown in pET20b case. (no one colony)
In case of mock-pET20a vector transformed very well.
Simplest explanation - wrong antibiotic used on the pET20b case?
What's the problems? I never experieced transfomation problems.
Does anyone know how to solve the this problems?
Thanks a lot !!
Genie,
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Eric T. Larson, PhD
Biomolecular Structure Center
Department of Biochemistry
Box 357742
University of Washington
Seattle, WA 98195
email: larso...@u.washington.edu
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