Hi Brittney,
DNA is pretty standard so the restraints should be in the dictionary. Perhaps the DNA in your model has non-standard residue names (PDBv2). Are your bases called DT, DA, etc? Do your atom names have "*" or "'"? Cheers, Robbie ________________________________ > Date: Tue, 26 Jul 2011 12:45:08 -0400 > From: [email protected] > Subject: [ccp4bb] Creating a non-minimal mmCIF dictionary for DNA? > To: [email protected] > > I am trying to refine a protein-DNA structure, and I am having > difficulties refining the DNA using COOT. I used the ccp4i Phaser > program to molecular replace the protein and DNA simultaneously (there > are solved crystal structures of the free protein and a similar DNA > bound to another protein), but I am only able to manually refine the > protein in COOT. If I try to refine the DNA, a window pops up with the > following statement: No Restraints Found! Non-existent or minimal > description of restrained residues. How do I create a non-minimal mmCIF > dictionary for just the DNA? I am relatively new to crystallography > and would appreciate any guidance. > > Brittney >
