Hi everyone, I'm currently working on refining a structure and have found that including tls parameters improves the r-factors and map quality. From previous threads I've seen that using TLS refinement leads to B values that are artificially low. I ran tlsanl as suggested and the average B value increased back to a reasonable value. What I'm wondering now is when I go to deposit the structure in the PDB do I use the output from tlsanl that includes anisoU values or do I use the refmac output and include details of the refinement procedure. This is my first time refining using TLS, so any help would be greatly appreciated. Sorry if this question has already been answered but I couldn't find a similar topic.
Jeff
