Hi Anita,
Won't harm if you put it on crystallization tray. You never know what
these proteins might do.
Allan
Quoting Zheng Zhou <zhengzho...@gmail.com>:
Hi, Anita
If you could find a way to test the elute's activity/binding to its'
substrat/cofactor, then you will learn much more about your target. If the
function assay is elusive, you could try superose column (5KDa-5MKDa). Does
your light scattering tell you about the estimated size and MW?
Best,
Joe
On Sat, Aug 27, 2011 at 1:29 AM, anita p <crystals...@gmail.com> wrote:
Hi Yury,
I have done dynamic light scattering and it shows its polydispersed.
Please let me know if it is still ok for setting trays.
reg.
anita
On Fri, Aug 26, 2011 at 10:21 PM, Yuriy Patskovsky <
yuriy.patskov...@einstein.yu.edu> wrote:
Anita,
an assembly may be quite large - I would check it somehow, maybe by light
scattering or centrifugation
Good luck
Yury
------------------------------
*From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of anita p
[crystals...@gmail.com]
*Sent:* Friday, August 26, 2011 3:03 AM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] Protein aggregation and crystallization
Hi All,
I am working on a protein which has a membrane spanning region and as
cytosolic domain.I have made various deletion constructs of the
protein, so
that I can have a crystallizable fragment. There is no homologues
mentioned
in the pdb for this protein.
All of these constructs are purified successfully but when concentrated
and loaded on a gel filtration column Superdex-200, they elute in the void
volume. But the proteins donot precipitate out.... !!
Is it worth while to go ahead for crystallization trials??
Any other suggestion is most welcome.
Thanks
Anita
--
Allan Pang
PhD Student
G35 Joseph Priestley Building
Queen Mary University of London
London
E1 4NS
Phone number: 02078828480
