Hi all I am working with a human protein (not membrane protein) which is forming gel at higher concentration. I am not able to concentrate the protein even more than 7 mg/ml. It become like jelly which is hard to pipette out.
I have gone through all the suggestions which you guys proposed very recently for another member. what i mean that i already have tried different temperature, pH, salt concentration, reducing agents (DTT, TCEF, in different concentration), stability analysis. But unfortunately nothing is working. One think i want to try to add some detergent to it. If you guys have any experience about it then please suggest the name and/or kind of detergent for the protein. Any other suggestion also appreciated which might help. cheers M. Obayed Ullah E.mail: obayed.o...@yahoo.com obayed.o...@gmail.com
