The idea is not at all crazy. In a sense it is quite similar to
Stoichiometric variation screening* if you consider that the lattice of
the crystallized subunit may contain planes
that might be conserved in the crystal of your hope for 3 protein complex.
*Stura, E.A., Graille, M., Taussig, M.J., Sutton, B.J. Gore, M.G.,
Silverman, G.J., Charbonnier, J.-B. (2001)
Crystallization of macromolecular complexes: Stoichiometric variation
screening. J. Cryst. Growth 232:580-590.
Yet if the size discrepancy is quite large, the chances that will work
will be quite slim.
Good luck,
Enrico.
On Wed, 21 Sep 2011 19:04:43 +0200, Peter Hsu <hsuu...@u.washington.edu>
wrote:
Hi all,
I've been trying to crystallize a 3 protein complex recently with little
success. However, crystals of each subunit have previously been
crystallized. I was wondering if any one knows of any
literature/experiences where people have used seeds from an individual
subunit to seed for a complex and succeeded? Or is this just a crazy/bad
idea?
Thanks in advance for any input.
Peter
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
