At this resolution you may/ should be able to find anamalous scatterers
using the anom signal from P and S. the SHELXC/D/(E) package is very
good at this!
Once you have the sites for the heavier atoms I would expect most direct
methods programs couild extend that sub-structure.
Certainly ACORN seems to work without resolution limits, and give
excellent final maps.
Eleanor
On 09/24/2011 04:03 AM, Fan, Hai-fu wrote:
Dear Yuri,
If you have located all the heavy atoms (sulfur and phosphor) correctly, you
could try sulfur-SAD phasing using the program OASIS. This program has a
record of solving a 1206 residues protein with SAD signals from 22 sulfur
atoms scattered under Cu-Ka radiation and a record of solving a 213 residues
protein with SAD signals from 2 sulfur atoms scattered under Cr-Ka
radiation.
By the way please note that the OASIS in CCP4 6.2.x is not the uptodate
version. You can get a better version from http://cryst.iphy.ac.cn. The
latest version will be available on the website in the coming October.
Best regards,
Hai-fu
On Sat, Sep 24, 2011 at 2:49 AM, Yuri Pompeu<[email protected]> wrote:
Hello everyone,
I have a data set>99% completeness to 1.15A
This is a 400 amino acid long protein and it has 7 Met (Sulfur peaks around
20sigma)
And a tightly bound phosphate (P peak around 22sigma)
Could I try and solve this directly or is it crazy idea?
If so what program should I try?
thanks
Yuri
