I actually looked at an EtBr MSDS a while ago, and was shocked at how benign it was. I also heard from someone that they used to feed it to Argentinian cows routinely a few years back...
JPK On Sat, Oct 1, 2011 at 1:56 AM, James Stroud <[email protected]> wrote: > If you can reproduce the crystals and have the material > > 1. Harvest several large crystals. > 2. Make several transfers to fresh mother liquor to wash. > 3. Dissolve in DNA loading dye without SDS > 4. Run on a native gel (e.g. 6% polyacrylamide, 0.5XTBE, etc.). > 5. Include positive control lanes for protein, DNA, and complex. > 6. Stain with ETBr. Take a picture. > 7. Wash out the ethidium in accordance with state, local, federal, UN, laws, > filling out the proper documentation ad nauseum. Take a safety class just to > be sure you didn't miss something. Hug a bureaucrat. > 8. Stain with coomassie. Take a picture. > > That should tell you more than you need to know. > > James > > On Sep 30, 2011, at 9:36 PM, zq deng wrote: > >> Hi all, >> . >> recently,I got a crystal of protein-DNA crystal.i used silver stainto prove >> that it is a protein crystal.Does anyone have method to detect if there is >> DNA in the crystal. >> any suggestion will be appreciated. >> >> Regards, >> deng > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: [email protected] *******************************************
